Cell genomics

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Stem Genomics provides a new test to assess the genomic integrity of stem cells. This innovative test is done using the supernatant collected from your cell cultures that has been shipped at room temperature.
This rapid, sensitive, conclusive and cost-effective test allows the routine monitoring of the genomic integrity of cultured pluripotent stem cells.

iCS-digital TM PSC
The in Culture Supernatant – digital PCR (iCS-digital TM) PSC test detects more than 90% of recurrent genomic abnormalities in human pluripotent stem cells (hPSCs), i.e. human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

About

Stem Genomics

Stem Genomics develops a technology invented by Prof John De Vos and Dr Said Assou, researchers at the Institute for Regenerative Medicine & Biotherapy (IRMB), University of Montpellier, and co-founders of the company. Located at the heart of the University Hospital Centre of Montpellier, IRMB houses research groups working on basic stem cell research and on innovative applications for regenerative therapy, with the aim of facilitating the transfer from research to clinical applications. Stem Genomics is located at the IRMB Cyborg business incubator and works in close collaboration with IRMB researchers. Stem Genomics has emerged from a prestigious scientific environment.

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The Ed-digital PCR test directly informs on the presence and number of transgenes copies  inserted in the genome of CRISPR-edited cells
Thanks to its flexibility and ability to achieve precise targeting, the CRISPR system is a powerful gene editing tool for various molecular biology applications. Precise control of the transgene integration is a major challenge for human cell research biosafety. Despite the improvement of CRISPR specificity, a significant proportion of transgenes integrates at unintended sites. These off-target insertions can cause genomic alterations that may disrupt normal cell functions with dramatic consequences on research outcomes. Therefore, it is crucial to verify the transgene presence at the desired genome site using PCR or sequencing, and also to confirm the absence of any possible additional unwanted insertion using Ed-digital, Stem Genomics digital PCR test for transgene copy number quantification. Ed-digital services The Ed-digital test allows the detection of the number of transgene copies present in the genome of gene-edited cells using droplet digital PCR. Ed-digital targets common resistance cassettes (puromycin, neomycin) as well as the fluorescent reporter gene mCherry. Examples of Ed-digital applications in gene-edited induced pluripotent stem cell (iPSC) lines Analysis of transgene integration in iPSC lines (graph on the left): A. Determination of the number of neomycin gene copies in two iPSC samples. The first sample carries two copies and the second one single copy of the transgene. B. Determination of puromycin gene copies in two iPSC samples. The first one carries two copies and the other one displays mosaic integration (n=2.5) of the transgene. Discover the test and request a quote

iCS-digital: A method for monitoring hPSC genome integrity that detects more than 90% of recurrent genomic abnormalities. The test is rapid (2-3 days), convenient (full service), straightforward, and can be performed using cell culture supernatant. It is particularly suitable for the routine monitoring of cell lines in culture and for screening genetically stable clones (e.g., CRISPR-edited iPSC clones, patient-derived iPSC clones…).