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Genomic abnormalities can lead to the potential development of tumors in patients and reduce the efficacy of MSC treatments (Neri, 2019). Traditional G-Banding karyotyping is helpful in providing an exhaustive structural and numerical variant analysis. However, it can be long and difficult to organize. This led Stem Genomics to develop a specific solution for research scientists working on hMSCs.
iCS-digital™ MSC has been designed to capture 80% of abnormalities found in MSCs.
We carried out an exhaustive meta-analysis of genomic abnormalities and referenced about 300 genetic abnormalities in hMSC lines. We then used this data to design our own assay: a set of 24 probes optimized to target all human chromosomes.
Our digital PCR-based solution enables rapid and cost-effective screening for chromosomal abnormalities, delivering results in just 3 days.
iCS-digital™ MSC can also complement a process already in place and bring an extra layer of precision to your QC.
Human MSCs
After isolation
During cell expansion
Before banking
gDNA
Cell pellets
Room temperature
Dry ice
>80% of recurrent abnormalities
>20% (depending on sample quality)
2-3 days after sample reception
Recommended guidelines in terms of genomic stability advise checking the cells during their time in culture but also screening clones after any stressing process (reprogramming, gene editing, etc.) and during banking. It is also advisable when a new line is acquired.
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Amplification
Amplification
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1682 rue de la Valsière
34 790 Grabels
5501 Fortunes Ridge Drive,
Suite O,
Durham, NC 27713
Get a comprehensive review of the factors to consider when culturing hPSCs and explore strategies to maximize your chances for genetic stability.
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Join us LIVE for a Q&A session on the latest iCS-digital™ PSC upgrade.
Find out what it means for you and ask all your questions
Juline VINCENT our R&D Project Manager and Digital PCR expert will answer you LIVE.
SAVE THE DATE: Thursday, November 7, 6pm CET/9am PDT/12 pm EDT.
Join us LIVE for our webinar “Genomic stability made easy” to discover how Stem Genomics has turned cutting-edge technologies into easy testing solutions accessible to most labs.
Get expert opinions and user perspectives on our genomic stability assays in the context of iPSC research.
Happening on Tuesday, November 14, 6 pm CET
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