Ready-to-use DNA controls
for your iCS-digital™ PSC kits

Take the uncertainty out of the equation when performing digital PCR tests in-house.

Have you ever been in a situation where the results obtained from your digital PCR experiment did not make sense and you wished you had proper reference standards in place?

To help you regain control over your experiments and increase your quality standards, Stem Genomics has developed a range of ready-to-use positive controls. They are designed to help you understand any variabilities and identify potential errors in your workflow.

This panel of human controls can be used with our iCS-digital™ PSC kit range. It includes abnormalities found on all the targeted positions in our 24-probe or 20q kits, ie. the most frequent abnormalities found in human pluripotent stem cells. (ref. Assou 2020)

These controls enable you to:

  • Check the conservation of reagents,
  • Check that no mix has been forgotten or switched during the experiment,
  • Determine whether the issue is caused by the reagent or the sample quality in the event of a sample with a low quality 2D-cluster well,
  • Increase the chances of success of your dPCR protocol.

DNA control specifications at a glance

Positive controls for the most recurrent abnormalities found in hPSCs

Characteristics:

  • Made with synthetic DNA mixed with DNA extracted from blood
  • Ready-to-use at 5 ng/µL

Indications:

Match the control with the corresponding mix. For example, Control 1 must be analyzed with MIX 1, Control 2 with MIX 2, etc …

Product

Quantity (volume)

Content

Control 1

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with Xp, 12p and 20q anomalies.

Control 2

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 18q, 9q and 17q anomalies.

Control 3

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 1q, 17p and 5q anomalies.

Control 4

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 11p, 13q and 7q anomalies.

Control 5

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 1p, 4q and 3p anomalies.

Control 6

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 19p, 14q and 8q anomalies.

Control 7

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 15q, 6q and 7p anomalies.

Control 8

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 22q, 16q and 2q anomalies.

You can choose between using the full panel of controls (all the controls from 1 to 8), or buying them “à la carte”.

Single control for the most recurrent abnormality found in hPSCs

Characteristics:

  • Made with synthetic DNA mixed with DNA extracted from blood
  • Ready-to-use at 5 ng/µL

Product

Quantity (volume)

Content

Control 20q-only

20 reactions  (60 µL – 5 ng/µL)

Positive control DNA (male) with 20q anomaly.

Additional controls for use with our kits

Product

Quantity (volume)

Content

Normal control

10 tests  (240 µL – 5 ng/µL)

Normal control DNA (male) with CNV=1 at the ChrXp region and CNV=2 in the other 23 regions.

DNA control descriptions

White paper: Ensuring genetic integrity in human Pluripotent Stem Cells : challenges and solutions

Get a comprehensive review of the factors to consider when culturing hPSCs and explore strategies to maximize your chances for genetic stability.

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Join us LIVE for a Q&A session on the latest iCS-digital™ PSC upgrade.

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Juline VINCENT our R&D Project Manager and Digital PCR expert will answer you LIVE.

SAVE THE DATE: Thursday, November 7, 6pm CET/9am PDT/12 pm EDT.

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