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Human Mesenchymal Stromal Cells (hMSCs) have wide-ranging therapeutic applications but require robust quality control in order to ensure reproducibility of research or a quality end product. Challenges like genomic instability, cell line cross-contamination or mycoplasma need to be addressed effectively.
Stem Genomics recognizes the variety of needs coming from MSC laboratories. We have therefore designed a broad offer for hMSC scientists that takes into account varying budget, performance and timescale expectations.
Detect over 80% of recurrent abnormalities in hMSCs in record-breaking time !
A G-Banding karyotype service optimized for MSCs
Look deeper into any human cell type and detect targeted SNVs and Indels
Combine 3 essential tests and get results in just 3 days.
Ensure consistent cell line identity throughout your culture
Confidently detect mycoplasma in any human cell culture
Genomic stability in MSCs can be altered during their time in culture, raising issues. It can significantly reduce the cells’ differentiation potential, or have a detrimental impact on their therapeutic properties. It can also potentially impact patient safety in the long term (Neri, 2019).
G-Banding karyotyping is the gold standard for detecting abnormalities in mesenchymal stromal cells and provides a comprehensive assessment (balanced and unbalanced translocations, deletions, insertions and inversions).
However, if you need a faster solution at a lower cost, digital PCR can be an interesting alternative.
Finally, if you want to go further in genomic stability testing, a more comprehensive approach including both SNV and CNV detection is required that only the latest sequencing technologies such as NGS (Next Generation Sequencing) can deliver. This powerful technology will allow the detection of SNVs in 361 targeted locations and CNVs across the genome that may not have been previously considered or that could harbor unexpected variations. This comprehensive and unbiased view adds significant information on the genomic stability of your cells.
Detect over 80% of recurrent abnormalities in hMSCs in record-breaking time !
A G-Banding karyotype service optimized for MSCs
Look deeper into any human cell type and detect targeted SNVs and Indels
Mislabeling or cross-contamination create risks of misidentification of cell lines. This happens more often than one might think: The International Cell Line Authentication Committee (ICLAC) keeps a registry of misidentified cell lines and to date, estimates that as many as 1/5 to 1/3 of known cell lines are wrongly identified. As of April 2024, their register of Misidentified Cell Lines holds records of 545 misidentified cell lines with no known authentic stock. It is therefore critical to check the cell line identity regularly throughout the time in culture. In terms of methodology, STR has been formally developed into an internationally recognized and accepted consensus standard for human cell line authentication and is the approach recommended by the ISSCR.
Mycoplasma contamination can alter the capacity of pluripotent stem cells to differentiate properly and wreak havoc in labs. Unlike other bacteria, mycoplasma cannot be seen by observation only and can take months to get rid of. It is therefore strongly recommended to regularly check for possible contamination and ensure the complete absence of mycoplasma in your cell culture.
The following guidelines are based on our extensive experience working with hMSC research scientists and recognized guidelines such as the ISSCR Standards for Human Stem Cell use in Research.
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Amplification
Amplification
Mesenchymal Stromal Cells (MSCs) are multipotent progenitor cells possessing self-renewal ability (limited in vitro) and differentiation potential into mesenchymal lineages, according to the International Society for Cell and Gene Therapy (ISCT).
They are also known as stromal/stem cells, medicinal signalling cells or in relation with their source, for instance “adipose-derived regenerative cells”.
In the 1960s, Friedenstein et al. identified a population of fibroblast-like cells that formed clonal colonies in vitro (CFU-F, Colony Forming Unit-Fibroblast) Friedenstein’s observations allowed for the discovery of a specific type of cell, currently referred to as mesenchymal stem cells (MSCs). MSCs are primary, non-specialized, non-hematopoietic, plastic-adherent cells with great proliferation potential and the capacity for self-renewal and differentiation. Source: Musiał-Wysocka A, Kot M, Majka M. The Pros and Cons of Mesenchymal Stem Cell-Based Therapies. Cell Transplant. 2019 Jul.
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Join us LIVE for a Q&A session on the latest iCS-digital™ PSC upgrade.
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Juline VINCENT our R&D Project Manager and Digital PCR expert will answer you LIVE.
SAVE THE DATE: Thursday, November 7, 6pm CET/9am PDT/12 pm EDT.
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