Myco-digital

The Myco-digital PCR test allows the fast and accurate detection of mycoplasma contamination in cell cultures.

Myco-digital workflow
Cell types

Any cells

Sensitivity

≥ 20 genome copies / PCR reaction

Samples

Cell culture supernatant (≥ 500 µL)

Shipment

Room temperature

Detection

> 60 mycoplasma species, including the 6 species responsible for 90-95% of cell culture infections

Time

2-3 days after sample reception

Digital PCR for the detection of mycoplasma contamination

Monitoring cell cultures for potential mycoplasma contaminations is mandatory to ensure the cell line safety and for high-quality research.
 
The Myco-digital test is a fast and accurate digital PCR-based test for mycoplasma detection in cell culture.
 
It has been precisely designed to target the conserved 16S ribosomal RNA coding region of the mycoplasma genome.
 
Based on sequence homology, the Myco-digital test can detect more than 60 species, including the six species responsible for about 90-95% of all cell culture mycoplasma infections (Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma hyorhinis, and Mycoplasma orale).
 

Myco-digital results

Myco-digital results

A) Digital PCR 2D plots. Each dot represents one PCR reaction droplet. Top panel: positive control with a cluster of droplets with mycoplasma DNA (blue) and negative droplets (grey). Bottom panel: negative control with only negative droplets (grey).
B) Example of a Myco-digital results report.

As the Myco-digital test uses the same supernatant sampling method,
it can be run alongside Stem Genomics iCS-digital TM tests.

Myco-digital